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HOC Grow Methodology

Tissue Culture

House of Cultivar Tissue Culture

HOC utilizes an advanced Tissue Culture and Micro Propagation laboratory to ensure the optimal success of all its different genetic cultivars

Summary

Tissue culture as a method of micropropagation first came to the fore in the 1950s in the orchid industry. In the decades since, the practice has been adopted by just about every other agricultural crop—from flowers, fruits and vegetables to hops, hay and, now, cannabis. Plant tissue culture is a collection of techniques harnessed to maintain or grow plant cells, tissues or organs under sterile conditions in culture media, a rich blend of ingredients that promote plant-cell growth. These ingredients can include macronutrients, micronutrients, vitamins, agar and other elements like activated charcoal. The process is widely used to produce plantlet clones using a method known as micropropagation. Tissue culture is seen as an important technology for the production of disease-free, high quality planting material and the rapid production of many uniform plants. It’s also used to preserve plant genetics.
Micropropagation, which is a form of tissue culture, increases the amount of planting material to facilitate distribution and large scale planting. In this way, thousands of copies of a plant can be produced in a limited amount of donor mother plant material. Micropropagated plants are observed to establish more quickly, grow more vigorously and are taller, have a more uniform production cycle than conventional propagules.

HOC tissue culture lab

GENETIC CONSISTENCY BENEFITS

Just as every person is different and unique, so is each plant. Some have traits like better color, yield, or pest resistance. For years, scientists have looked for methods to allow them to make exact copies of these superior individuals.
Plants usually reproduce by forming seeds through sexual reproduction. That is, egg cells in the flowers are fertilized by pollen from the stamens of the plants. Each of these sexual cells contains genetic material in the form of DNA. During sexual reproduction, DNA from both parents is combined in new and unpredictable ways, creating unique plants.
This unpredictability is a problem for plant breeders as it can take several years of careful greenhouse work to breed a plant with desirable characteristics. Many of us think that all plants grow from seeds but most come from a mother plant and clone. Cannabis cultivators, including HOC, have long relied on cloning as a way to propagate plant inventory and preserve their genetics. But cloning—taking a rooted cutting of a mother plant to grow a genetically identical plant— also has it issues with maintaining plant sterility, health, and epigenetic changes. In addition, keeping mother plants in a conventional setting takes lots of space, labor, water, nutrients and other resources. For all these reasons, we have supplemented cloning propagation with a far superior method to propagate and preserve a plant’s genetics: tissue culture.

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